The Deputy First Minister is expected to set out the SNP's position on membership of the European Union after independence in a statement at the Scottish Parliament.
Nicola Sturgeon's appearance before MSPs on Thursday follows comments from the president of the European Commission, Jose Manuel Barroso, who suggested Scotland would have to reapply.
The Scottish Government position is at odds with Mr Barroso's view as head of the EU's ruling body.
The SNP argues that Scotland will negotiate from within the UK, the member state, after a Yes vote in 2014 but before formal independence.
Mr Barroso has said that if a country becomes independent, it is a new state and has to negotiate with the EU.
No formal legal opinion has been published and there is no precedent for part of a member state becoming an EU member in its own right.
Scottish Conservatives called for a statement to be made at Holyrood.
John Lamont, the party's chief whip, said: "This a matter of huge importance and it's only right the Scottish Government explains to the Parliament and the people why it thinks Mr Barroso is incorrect.
"If the SNP doesn't disagree with the EC president we need to know why, for the past year, it has attempted to dupe the Scottish people into thinking a separate Scotland would be simply waved into the EU."